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Herpes genitalis is probably more prominent today than any other sexually transmitted disease. Type 2 virus accounts for most infections seen; however, type 1 infections are reported more and more often as time pases. Viral isolation is the most sensitive and specific technique for esterblishing the diagnosis of herpes virus(HSV) infection. In this study the positivity rate of HSV culture was investigated in asymptomatic gravidas and gynecologic patients. In patients with positive HSV cultures, the typing for HSV by direct immunofluo- rescent stain was carried out. The results were as follows; 1. The positivity rate of HSV culture in obstetric patients and gynecologic patients was 3.4%(2/58) and 20.0%(14/70), respectively. There was no significant difference as compared to 5.3%(1/19) of control group (p>0.05, p=0.2922). 2. Isolation rate was 31.8%(7/22) in gynecologic patients with ulcer or vesicle-like lesion and 15.4%(2/13) in patients with cervical cancer. However, all of them had no statistical significance as compared to control group (p=0.1189, p=0.5649). 3. Over 88% (16/18) of HSV isolates were recovered within three days, 72.2%(13/18) by two days and 27.8%(5/18) by one day. 4. Of 17 patients with positive cultures, HSV type 1, HSV type 2 and mixed infections was 17.7% (3/17), 94.3% (16/17) and 11.8%(2/17), respectively.
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